polA [2019-01-30 09:29:11]

DNA polymerase I, required for repair of UV- or MMS-induced DNA damage, processing of Okazaki fragments

Locus: BSU29090

Isoelectric point: 4.92

Molecular weight: 98.89 kDa

Protein length: 880 aa

Gene length: 2643 bp

Function: DNA replication

Product: DNA polymerase I

Essential: no

E.C.: 2.7.7.7

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA replication
• Information processing → Genetics → DNA repair/ recombination
• Information processing → Genetics → DNA repair/ recombination → Other proteins

Gene

Coordinates: 2,973,182 2,975,824

Phenotypes of a mutant

• strongly reduced stationary phase mutagenesis PubMed
• a polA exoR double mutant is not viable (synthetic lethality) PubMed
• a rnhC polA double mutant is not viable at 25°C PubMed

The protein

Catalyzed reaction/ biological activity

• has DNA polymerase and 5'-3' exonuclease activity, but no 3'-5' exonuclease activity PubMed
• maturation of Okazaki fragments (together with RNase HIII) PubMed

Protein family

• DNA polymerase type-A family (according to Swiss-Prot)

Paralogous protein(s)

• ExoR:

Structure

• 1XWL (large fragment, corresponds to AA 310 to 880, Geobacillus stearothermophilus)

Expression and Regulation

Operons

• Genes: polA-mutM-ytaF-ytaG
  Description: PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

Biological materials

Mutant

• BKE29090 (polA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCAATTTCCTCCTAAGA, downstream forward: _UP4_TAAACAGAGATAGGAAGTGA
• BKK29090 (polA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCAATTTCCTCCTAAGA, downstream forward: _UP4_TAAACAGAGATAGGAAGTGA

References

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